An enzymatic reaction using glucose oxidase was applied for in situ production of hydrogen peroxide for use in simultaneously Fenton's reaction in decolorization of malachite green. It was found that decolorization rate increased by increasing of glucose concentration from 0.2 g/L to 1.5 g/L. Decolorization rate showed different behaviors versus temperature changes. Initial rate of decolorizati...

Two biological approaches for decolorization of azo sulfonated dyes have been compared: reductive decolorization with the ascomycete yeast Issatchenkia occidentalis and enzymatic oxidative decolorization with Trametes villosa laccase alone or in the presence of the mediator 1-hydroxybenzotriazole. The redox potential difference between the biological cofactor involved in the reductive activity ...

The aim of this study is the evaluation of the enzymatic action of the ligninolytic enzyme manganese peroxidase (MnP), through a suitable addition of H(2)O(2), as a feasible system for the in vitro degradation of complex structures. For this purpose, a highly recalcitrant polymeric dye (Poly R-478) was selected as a model compound. An amperometric technique was used to determine the H(2)O(2) re...

in this study, decolorization of the diazo dye, reactive black 5 (rb5) in a coprinus cinereus peroxidase-catalyzed reaction has been investigated. a bisubstate kinetic model for the reaction rate based on the ping-pong mechanism was assumed for the enzymatic decolorization. experimentswere conducted at different rb5 and hydrogen peroxideconcentrations in a batch mannerto estimate the intrinsic ...

BACKGROUND In recent years, enzymatic-assisted removal of hazardous dyes has been considered as an alternative and eco-friendly method compared to those of physicochemical techniques. The present study was designed in order to obtain the optimal condition for laccase-mediated (purified from the ascomycete Paraconiothyrium variabile) decolorization of Acid Blue 92; a monoazo dye, using response ...

Biological treatment, especially enzymatic methods, can be employed for effective and environmental- friendly treatment of dye effluents. Laccase, belonging to the blue multi-copper oxidases category, can oxidize a wide variety of substrates, especially synthetic dyes. In this study, laccase was used to biodegrade two azo dyes, i.e., Direct Red 23 and Acid Blue 92. Before conducting the exp...

Pycnoporus sanguineus is a white-rot basidiomycete that produces laccase as the only oxidoreductase; enzyme synthesis depends on cultivation variables, and fungal species and strain. Laccases have wide substrate specificity, oxidize a broad range of compounds, and show potential for use in dye decolorization. We evaluated laccase production in a recently isolated strain of P. sanguineus cultiva...

In the present investigation, four species of white rot fungi (Pleurotus), that is, P. flabellatus, P. florida, P. ostreatus and P. sajor-caju were used for decolorization of direct blue 14 (DB14). Among all four species of Pleurotus, P. flabellatus showed the fastest decolorization in petri plates on different concentration, that is, 200 mg/L, 400 mg/L, and 600 mg/L. All these four species wer...